3Q0H
Structure of T-cell immunoreceptor with immunoglobulin and ITIM domains (TIGIT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.951, 74.671, 43.346 |
| Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
| Resolution | 43.310 - 1.700 |
| R-factor | 0.2087 |
| Rwork | 0.207 |
| R-free | 0.23700 |
| Structure solution method | MIR |
| Starting model (for MR) | 1ikf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.678 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.038 | 0.027 | 0.535 |
| Number of reflections | 27916 | ||
| <I/σ(I)> | 16.4 | ||
| Completeness [%] | 99.7 | 94.5 | 100 |
| Redundancy | 7.3 | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 1.3M Ammonium Sulfate, 0.1M MES pH 6.0, Vapor diffusion, Sitting drop, temperature 298K |
