3PZV
C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.4586 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.359, 81.080, 114.270 |
| Unit cell angles | 90.00, 104.29, 90.00 |
Refinement procedure
| Resolution | 41.464 - 2.867 |
| R-factor | 0.2335 |
| Rwork | 0.230 |
| R-free | 0.29610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.261 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 3.000 |
| High resolution limit [Å] | 2.867 | 2.900 |
| Number of reflections | 30407 | |
| Completeness [%] | 97.1 | 83.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 20% PEG3350 200 mM sodium Nitrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
