3PZP
Human DNA polymerase kappa extending opposite a cis-syn thymine dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.136, 154.476, 217.854 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.670 - 3.336 |
| R-factor | 0.24389 |
| Rwork | 0.242 |
| R-free | 0.28778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.681 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.350 |
| High resolution limit [Å] | 3.230 | 6.950 | 3.230 |
| Number of reflections | 31594 | ||
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 99.4 | 99.4 | 95.3 |
| Redundancy | 4.8 | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 14% PEG 5000 monomethylether, 0.2M potassium acetate, 0.1M sodium chloride, 0.1M sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
