3PZ5
The crystal structure of AaLeuRS-CP1-D20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 106.143, 106.143, 81.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.500 |
| R-factor | 0.2151 |
| Rwork | 0.213 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.989 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15748 | |
| Completeness [%] | 79.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30 % PEG 4000, 0.1 M sodium citrate tribasic dihydrate at pH 5.6 and 0.2 M ammonium acetate , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
