3PVU
Bovine GRK2 in complex with Gbetagamma subunits and a selective kinase inhibitor (CMPD101)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.504, 73.502, 121.993 |
| Unit cell angles | 90.00, 115.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.480 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.5.0102) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.478 | 5.380 | 2.500 |
| Number of reflections | 51786 | ||
| <I/σ(I)> | 16.4 | ||
| Completeness [%] | 99.1 | 99.6 | 98.6 |
| Redundancy | 6.4 | 6.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 277 | 7% PEG3350, 200 mM NaCl, 100 mM MES pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
