3PU3
PHF2 Jumonji domain-NOG complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.910, 96.335, 66.373 |
| Unit cell angles | 90.00, 89.91, 90.00 |
Refinement procedure
| Resolution | 31.180 - 1.950 |
| R-factor | 0.2285 |
| Rwork | 0.226 |
| R-free | 0.29370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kv4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.180 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.085 | 0.472 |
| Number of reflections | 60290 | |
| <I/σ(I)> | 23.3 | 3.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 18-30% polyethylene glycol 3350 and 100 mM NaCitrate, pH 5.2-6.0, VAPOR DIFFUSION |
