3PQR
Crystal structure of Metarhodopsin II in complex with a C-terminal peptide derived from the Galpha subunit of transducin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 241.550, 241.550, 109.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.860 - 2.850 |
| Rwork | 0.217 |
| R-free | 0.24958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dqb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.865 | 3.000 |
| High resolution limit [Å] | 2.849 | 2.850 |
| Rmerge | 0.059 | 0.409 |
| Number of reflections | 28448 | |
| <I/σ(I)> | 15.9 | 2.9 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
