3PN5
Crystal structure of Arabidopsis thaliana petide deformylase 1B (AtPDF1B) G41Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.930, 56.930, 147.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.470 - 2.300 |
| R-factor | 0.305 |
| Rwork | 0.301 |
| R-free | 0.33800 |
| Structure solution method | rigid body |
| Starting model (for MR) | 3m6o |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 11253 | |
| <I/σ(I)> | 13.64 | 5.82 |
| Completeness [%] | 98.3 | 94 |
| Redundancy | 6.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG-3350, Zinc acetate 100mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
