3PLQ
Crystal structure of PKA type I regulatory subunit bound with Rp-8-Br-cAMPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.990, 66.990, 58.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.249 |
| R-free | 0.32750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FHI(Chain B) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.210 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 7036 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.5 | 298 | pH 6.5, hanging drop, temperature 298K |
