3PLA
Crystal structure of a catalytically active substrate-bound box C/D RNP from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 241.199, 241.199, 145.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 3.150 |
| R-factor | 0.24928 |
| Rwork | 0.248 |
| R-free | 0.27761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3ICX 3id6 3id5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.095 | 0.536 |
| Number of reflections | 70682 | |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 95.1 | 94.4 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2.0M (NH4)2SO4, 2% PEG 400, 10mM MgCl2, 0.1M HEPES-Na (pH 6.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
