3PKM
Crystal structure of Cas6 with its substrate RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 96.557, 96.557, 165.492 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.347 - 3.103 |
| R-factor | 0.3085 |
| Rwork | 0.276 |
| R-free | 0.31760 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 15227 | |
| Completeness [%] | 83.7 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 20 mM MgCl2, 50 mM NaHepes (pH7.0), 1.4 M Ammonium Sulfate, 4% polypropylene Glycol 400 (p400), 20 mM NiCl2, and 100 mM NaCl, vapor diffusion, hanging drop, temperature 303.0K |
