3PHX
OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with ISG15
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.520, 37.100, 84.350 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1421 |
| Rwork | 0.140 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.395 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.4_122)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 33549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% PEG8K, 0.2M zinc acetate, 0.1M MES sodium salt, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
