3PD9

X-ray structure of the ligand-binding core of GluA2 in complex with (R)-5-HPCA at 2.1 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	110
Detector technology	CCD
Collection date	2004-06-16
Detector	MAR CCD 165 mm
Wavelength(s)	0.8126
Spacegroup name	P 21 21 2
Unit cell lengths	99.391, 121.251, 47.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.415 - 2.100
R-factor	0.1798
Rwork	0.177
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nnp
RMSD bond length	0.007
RMSD bond angle	1.005
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.104	0.047	0.411
Number of reflections	34007
<I/σ(I)>	9.3
Completeness [%]	99.9	99.3	99.9
Redundancy	4.1	3.8	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	280	PEG4000, ammonium sulfate, acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K