3PB6
Crystal structure of the catalytic domain of human Golgi-resident glutaminyl cyclase at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.097, 68.527, 77.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.050 |
| R-factor | 0.13875 |
| Rwork | 0.138 |
| R-free | 0.15770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pb4 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.129 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.083 | 0.594 |
| Number of reflections | 127164 | |
| <I/σ(I)> | 37.4 | 3.5 |
| Completeness [%] | 96.4 | 96.7 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50%(v/v) PEG 200, 0.2M MgCl2, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
