3PB0
Characterisation of the first monomeric dihydrodipicolinate synthase variant reveals evolutionary insights
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9536 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.458, 131.472, 74.150 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 37.210 - 2.000 |
| R-factor | 0.1521 |
| Rwork | 0.151 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.286 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.160 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 82574 | |
| Completeness [%] | 91.1 | 79.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 200mM Lithium sulphate, 50% v/v PEG 400, 100mM sodium-citrate buffer, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
