3P7Z

Crystal structure of the Neurofibromin Sec14-PH module containing the patient derived mutation I1584V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	113.061, 113.061, 124.612
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.650
R-factor	0.2186
Rwork	0.216
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.144	2.870
High resolution limit [Å]	2.650	2.650
Number of reflections	24081
<I/σ(I)>		3.83
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	0.1M MES, 7.5% PEG 4000, 0.2M Na4P207, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K