3P56

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	212.238, 42.302, 186.950
Unit cell angles	90.00, 98.11, 90.00

Refinement procedure

Resolution	30.000 - 4.060
R-factor	0.3747
Rwork	0.374
R-free	0.37900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	re-refined PDB ID: 3KIO
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	4.250
High resolution limit [Å]	4.060	8.770	4.060
Rmerge	0.180	0.076	0.690
Number of reflections	13282
<I/σ(I)>	3.6
Completeness [%]	98.5	97.4	98.7
Redundancy	3.9	4.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	20% PEG 3350, 0.1M bis-Tris pH6.5, 0.2M KNO3, vapor diffusion, sitting drop, temperature 277.15K