3P4X
Helicase domain of reverse gyrase from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.259, 111.231, 129.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.032 - 2.410 |
R-factor | 0.1899 |
Rwork | 0.186 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oiy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.144 |
Data reduction software | XDS ((VERSION May 10) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.730 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.064 | 0.635 |
Number of reflections | 34066 | |
<I/σ(I)> | 12 | |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG3350, 0.2M Mg-formiate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |