3P11
anti-EGFR/HER3 Fab DL11 in complex with domains I-III of the HER3 extracellular region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.392, 48.397, 130.381 |
| Unit cell angles | 90.00, 127.71, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.700 |
| R-factor | 0.24922 |
| Rwork | 0.245 |
| R-free | 0.32299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 13436 |
| <I/σ(I)> | 12 |
| Completeness [%] | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 295 | PEG 1500, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
