3OTC
Crystal structure of human tRNAHis guanylyltransferase (Thg1)- Native II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 116.678, 116.678, 104.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.010 |
| Rwork | 0.257 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.232 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.120 |
| High resolution limit [Å] | 3.010 | 6.480 | 3.010 |
| Rmerge | 0.093 | 0.032 | 0.821 |
| Number of reflections | 16284 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.5 | 100 | 95.5 |
| Redundancy | 13.4 | 13.7 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | Na Formate, pH 7, hanging drop, temperature 298K |
