3ORF
Crystal Structure of Dihydropteridine Reductase from Dictyostelium discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 2.16 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.808, 129.898, 78.763 |
| Unit cell angles | 90.00, 99.99, 90.00 |
Refinement procedure
| Resolution | 36.960 - 2.160 |
| R-factor | 0.18771 |
| Rwork | 0.185 |
| R-free | 0.24014 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dhr |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.069 | 0.423 |
| Number of reflections | 46917 | |
| Completeness [%] | 93.4 | 96.4 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 17% PEG 3350, 0.1M bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
