3OQS

Crystal structure of importin-alpha bound to a CLIC4 NLS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	78.594, 89.558, 100.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.050 - 2.000
R-factor	0.199
Rwork	0.197
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ial
RMSD bond length	0.020
RMSD bond angle	1.672
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.739	21.051	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.041	0.776
Rmeas		0.045	0.871
Rpim		0.018	0.381
Total number of observations		10551	27080
Number of reflections	46758
<I/σ(I)>	12.2	30.8	1.8
Completeness [%]	96.7	97.2	80.3
Redundancy	6.8	6.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.7M sodium citrate, 10mM DTT, 70mM HEPES pH 7.4, vapor diffusion, hanging drop, temperature 293K