3OP0
Crystal structure of Cbl-c (Cbl-3) TKB domain in complex with EGFR pY1069 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-26 |
| Detector | ADSC Q315 3X3 CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 90.250, 90.250, 191.581 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.450 - 2.520 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 1FBV CHAIN A |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.543 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 2.660 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.130 | 0.745 |
| Number of reflections | 29742 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277.15 | 20% Jeffamine M-600, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
