Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.570, 99.570, 161.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 4.000 |
| R-factor | 0.28331 |
| Rwork | 0.280 |
| R-free | 0.36446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d4q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.072 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.167 | 0.311 |
| Number of reflections | 7341 | |
| <I/σ(I)> | 4.3 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.8 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 12% PEG 8K, 100 MM TRIS PH 8.0, 10% TACSIMATE, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
