3OJY
Crystal Structure of Human Complement Component C8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | MAR |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 139.575, 139.575, 127.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.690 - 2.510 |
| R-factor | 0.2532 |
| Rwork | 0.249 |
| R-free | 0.33740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rd7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.774 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.101 | 0.034 | 0.417 |
| Number of reflections | 44803 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 93.3 | 98.4 | 63.7 |
| Redundancy | 3.2 | 3.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 281 | 14-16% PEG 4K, 100mM TAPS pH 9.0, 400mM NaCl, 10mM SrCl2, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
