3OIJ

Crystal structure of Saccharomyces Cerevisiae Nep1/Emg1 bound to S-adenosylhomocysteine and 2 molecules of cognate RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00931
Spacegroup name	P 21 21 21
Unit cell lengths	83.377, 91.860, 96.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.886 - 3.000
R-factor	0.1901
Rwork	0.186
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3j
RMSD bond length	0.008
RMSD bond angle	1.290
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.000
High resolution limit [Å]	2.900	6.230	2.900
Rmerge	0.078	0.049	0.361
Number of reflections	16553
<I/σ(I)>	21.2
Completeness [%]	97.0	99.8	76.5
Redundancy	6.3	6.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.05M MES, 15mM MgS04, 7% PEG 4000, 20% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K