3OHT
Crystal Structure of Salmo Salar p38alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.780, 100.930, 67.890 |
| Unit cell angles | 90.00, 98.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w82 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.199 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP (10.2.23) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.054 | 0.418 |
| Number of reflections | 22427 | |
| <I/σ(I)> | 17.08 | 2.65 |
| Completeness [%] | 90.0 | 90.9 |
| Redundancy | 2.4 | 2.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.01 M MgCl2, 0.05 M Tris/HCl, 1.6 M NH4SO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
