3OB2
Crystal structure of c-Cbl TKB domain in complex with double phosphorylated EGFR peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER PROTEUM X8 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | BRUKER AXS MICROSTAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 122.771, 122.771, 55.425 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.850 - 2.100 |
| R-factor | 0.18449 |
| Rwork | 0.183 |
| R-free | 0.21732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bum |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.642 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 4.350 | 2.020 |
| Rmerge | 0.062 | 0.036 | 0.399 |
| Number of reflections | 30870 | ||
| <I/σ(I)> | 20.1 | ||
| Completeness [%] | 98.5 | 99.6 | 92.7 |
| Redundancy | 7.2 | 12.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | 20% (w/v) PEG 3350, 150mM NaK tartrate, 0.1 M Bis-Tris propane pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
