3O8O
Structure of phosphofructokinase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-28 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.1044 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 180.046, 186.205, 236.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.900 |
| R-factor | 0.2586 |
| Rwork | 0.259 |
| R-free | 0.30920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pfk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.619 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 172818 | |
| <I/σ(I)> | 15.13 | 1.75 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 6-10% PEG4000, 0.2 mM sodium acetate, 0.1 M MES buffer pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
