3O7U
Crystal structure of Cytosine Deaminase from Escherichia Coli complexed with zinc and phosphono-cytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 145.212, 145.212, 200.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.208 - 1.708 |
| R-factor | 0.1741 |
| Rwork | 0.173 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k6w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.017 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.208 |
| High resolution limit [Å] | 1.708 |
| Number of reflections | 86951 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% Pentaerythritol Propoxylate, 0.05M Hepes, 0.2M Potassium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
