3O7Q
Crystal structure of a Major Facilitator Superfamily (MFS) transporter, FucP, in the outward conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 119.460, 119.460, 98.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.643 - 3.143 |
R-factor | 0.2183 |
Rwork | 0.215 |
R-free | 0.27290 |
Structure solution method | SAD |
RMSD bond length | 0.047 |
RMSD bond angle | 1.352 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.643 | 3.250 |
High resolution limit [Å] | 3.143 | 3.140 |
Rmerge | 0.077 | 0.871 |
Number of reflections | 14221 | |
<I/σ(I)> | 21.12 | |
Completeness [%] | 98.3 | 99.4 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 34% PEG400, 0.1M cacodylate, 0.1M MgCl2, 0.05M NaF, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |