3O6W
Crystal structure of monomeric KlHxk1 in crystal form VIII (open state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.540, 93.590, 114.290 |
Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
Resolution | 29.120 - 1.480 |
R-factor | 0.1673 |
Rwork | 0.167 |
R-free | 0.18391 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o08 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.409 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.520 |
High resolution limit [Å] | 1.480 | 1.480 |
Number of reflections | 171952 | |
<I/σ(I)> | 21.7 | 2.5 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 292 | 1microL reservoir + 1microL protein, reservoir: 2.4M di-ammonia phosphate, 0.1M Ches pH 9.6, protein: 10mg/ml Klhxk1, 10mM Tris pH 7.4, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 1mM xylose, 1mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 292K |