3O4R
Crystal Structure of Human Dehydrogenase/Reductase (SDR family) member 4 (DHRS4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MAR225 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.070, 132.200, 133.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.290 - 1.700 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zat |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.290 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | |
| Number of reflections | 116449 | |
| <I/σ(I)> | 10.4 | 1.7 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 0.1M KSCN30% mPEG 2K, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
