3O47
Crystal structure of ARFGAP1-ARF1 fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 79.298, 129.161, 157.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.650 - 2.800 |
R-factor | 0.2072 |
Rwork | 0.206 |
R-free | 0.24330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 1HUR 3dwd |
RMSD bond length | 0.009 |
RMSD bond angle | 1.080 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.116 | 0.038 | 0.990 |
Number of reflections | 20250 | ||
<I/σ(I)> | 8.3 | ||
Completeness [%] | 99.9 | 99.7 | 99.8 |
Redundancy | 4.9 | 4.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 20% PEG-3350, 0.2M trilithium sulfate, pH not applicable, vapor diffusion, temperature 291K |