3NYN
Crystal Structure of G Protein-Coupled Receptor Kinase 6 in Complex with Sangivamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-08-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 61 |
Unit cell lengths | 154.590, 154.590, 207.914 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.720 |
R-factor | 0.22709 |
Rwork | 0.227 |
R-free | 0.23840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.064 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.310 | 2.700 |
Rmerge | 0.088 | 0.041 | 0.351 |
Number of reflections | 45850 | ||
<I/σ(I)> | 8.1 | ||
Completeness [%] | 59.4 | 98.9 | 1.2 |
Redundancy | 3.9 | 3.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 10 mg/ml GRK6 mixed 1:1 with well containing 1.9 M AMS and 100 mM Bis-Tris, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |