3NSJ
The X-ray crystal structure of lymphocyte perforin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953689 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.640, 109.800, 141.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.360 - 2.750 |
| R-factor | 0.1971 |
| Rwork | 0.196 |
| R-free | 0.21460 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.636 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.110 | 0.109 |
| Number of reflections | 32550 | |
| <I/σ(I)> | 18.8 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.5 | 16.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.5 M sodium acetate, 0.1 M imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
