3NQJ
Crystal structure of (CENP-A/H4)2 heterotetramer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2010-04-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 62.869, 62.869, 159.512 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.18249 |
Rwork | 0.180 |
R-free | 0.24141 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nqu |
RMSD bond length | 0.014 |
RMSD bond angle | 1.329 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.450 | 2.210 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.083 | 0.447 |
Number of reflections | 11585 | |
<I/σ(I)> | 23.39 | 4.3 |
Completeness [%] | 97.6 | 87.7 |
Redundancy | 8.22 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.17 M ammonium sulfate, 0.085 M sodium cacodylate (pH 6.5), 25.5% PEG-8000, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |