3NPA
Glycogen phosphorylase complexed with 2,5-dihydroxy-4-(beta-D-glucopyranosyl)-bromo-benzene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-05-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.702, 128.702, 116.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.969 |
| R-factor | 0.19194 |
| Rwork | 0.191 |
| R-free | 0.21271 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.969 | 1.969 |
| Rmerge | 0.065 | 0.298 |
| Number of reflections | 64845 | |
| <I/σ(I)> | 11.3 | 4.2 |
| Completeness [%] | 93.9 | 90 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 20 mg/mL protein in a buffer consisting of 10 mM BES, 0.5 mM EDTA, 3 mM DTT. T-state GPb crystals soaked with 20 mM inhibitor for 6 hrs, pH 6.7, SMALL TUBES, temperature 289K |
