3NMX
Crystal structure of APC complexed with Asef
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-04 |
| Wavelength(s) | 0.97939 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.790, 92.180, 107.840 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.20987 |
| Rwork | 0.208 |
| R-free | 0.25435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.823 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.068 | 0.038 | 0.276 |
| Number of reflections | 60878 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 95.1 | 99.5 | 72.2 |
| Redundancy | 7.1 | 7.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 287 | 25% PEG 1500, 0.1M NaCl, pH 9.0, vapor diffusion, hanging drop, temperature 287K |
