3NLB
Novel kinase profile highlights the temporal basis of context dependent checkpoint pathways to cell death
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.885, 65.710, 54.420 |
| Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
| Resolution | 27.940 - 1.900 |
| R-factor | 0.18382 |
| Rwork | 0.181 |
| R-free | 0.24108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.060 |
| Total number of observations | 184416 |
| Number of reflections | 24614 |
| Completeness [%] | 87.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
