3NHE

High Resolution Structure (1.26A) of USP2a in Complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	102.771, 53.961, 74.719
Unit cell angles	90.00, 107.71, 90.00

Refinement procedure

Resolution	19.180 - 1.260
R-factor	0.1697
Rwork	0.169
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hd5 2ibi
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.310
High resolution limit [Å]	1.260	1.260
Rmerge	0.066	0.258
Number of reflections	103417
<I/σ(I)>	12.6
Completeness [%]	98.0	88.1
Redundancy	3.5	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	295	5% PEG4000, 0.1M Hepes, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K