3NGB
Crystal structure of broadly and potently neutralizing antibody VRC01 in complex with HIV-1 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.82656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.630, 98.275, 205.256 |
| Unit cell angles | 90.00, 99.68, 90.00 |
Refinement procedure
| Resolution | 49.733 - 2.680 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3idx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.680 |
| Number of reflections | 97150 |
| <I/σ(I)> | 18.6 |
| Completeness [%] | 94.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 10% PEG 4000, 9% Isopropanol, 0.1M Ammonium Sulfate, 0.1M Tris/Cl-, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
