3NAQ
Apo-form of NAD-dependent formate dehydrogenase from higher-plant Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.330, 106.230, 106.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.160 - 1.700 |
R-factor | 0.16894 |
Rwork | 0.167 |
R-free | 0.19718 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.589 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.160 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.323 |
Number of reflections | 87821 | |
<I/σ(I)> | 31.25 | 5.36 |
Completeness [%] | 95.9 | 90.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein solution (2ul): 6 mg/ml FDH, 0.1M Na2HPO4, pH 7.0, 10 mM EDTA. Reservoir solution (2ul): 0.1M Bis-Tris, pH 6.0, 2.1M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |