3N45
Human FPPS complex with FBS_04 and zoledronic acid/MG2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | MARCCD165 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.753, 110.753, 67.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.030 - 1.880 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | APRV |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.079 | 0.405 |
| Number of reflections | 34881 | 4032 |
| <I/σ(I)> | 16.62 | 7.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14.3 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
