3N3B

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with Reduced NrdI with a Trapped Peroxide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	113
Detector technology	CCD
Collection date	2009-12-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0781
Spacegroup name	P 21 21 21
Unit cell lengths	75.723, 91.348, 144.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.560 - 2.360
R-factor	0.236
Rwork	0.234
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n39
RMSD bond length	0.006
RMSD bond angle	0.819
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.560
High resolution limit [Å]	2.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1