3MZG
Crystal structure of a human prolactin receptor antagonist in complex with the extracellular domain of the human prolactin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.874, 123.874, 72.468 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.070 - 2.100 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.22975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHIMERA OF PDB ENTRIES 3D48 2q98 AND 1BP3 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40530 | |
| <I/σ(I)> | 23.1 | 3.7 |
| Completeness [%] | 94.0 | 99.3 |
| Redundancy | 9.5 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 291 | 3.7 M NaCl; 0.1M Hepes pH 7.5, 600nL + 100 nL drops, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
