3MY1
Structure of CDK9/cyclinT1 in complex with DRB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00550 |
| Spacegroup name | H 3 |
| Unit cell lengths | 173.789, 173.789, 99.221 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.476 - 2.800 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.21420 |
| Structure solution method | Rigid body refinement |
| Starting model (for MR) | 3blh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.490 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.597 |
| Number of reflections | 27472 | |
| <I/σ(I)> | 6.87 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 277 | 12% PEG 1000, 100mM sodium/potassium phosphate at pH 6.2, 500mM NaCl, 2mM TCEP as a reservoir solution, EVAPORATION, temperature 277K |
