3MUZ
E.Coli (lacZ) beta-galactosidase (R599A) in complex with IPTG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 151.830, 163.280, 204.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.420 - 1.900 |
Rwork | 0.219 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dp0 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.420 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.116 | 0.609 |
Number of reflections | 56045 | |
<I/σ(I)> | 9.3 | 2.7 |
Completeness [%] | 98.7 | 97.7 |
Redundancy | 6.05 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | PEG8000, NaCl, MgCl2, DTT, Bis-Tris, pH6.5, HANGING DROP AT 288K, VAPOR DIFFUSION, HANGING DROP |