3MTC
Crystal Structure of INPP5B in complex with phosphatidylinositol 4-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97908 |
Spacegroup name | P 21 3 |
Unit cell lengths | 133.761, 133.761, 133.761 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.290 - 2.400 |
R-factor | 0.17234 |
Rwork | 0.171 |
R-free | 0.20544 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i9y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.278 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.510 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.091 | 0.698 |
Number of reflections | 31380 | |
<I/σ(I)> | 11.1 | 2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 22% polyacrylic acid, 0.02M magnesium chloride, 0.1M hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |