3MP7
Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-12-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97966, 0.97958, 0.96863 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.240, 141.570, 235.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.430 - 2.900 |
R-factor | 0.28 |
Rwork | 0.277 |
R-free | 0.31700 |
Structure solution method | MAD |
Starting model (for MR) | 1rh5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | ELVES |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 23475 |
<I/σ(I)> | 9.8 |
Completeness [%] | 98.0 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 273 | PEG4000-PEG8000 15-25%, MES 100mM pH 6.2, Ca or Mg Acetate 50-200mM, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 273 | PEG4000-PEG8000 15-25%, MES 100mM pH 6.2, Ca or Mg Acetate 50-200mM, VAPOR DIFFUSION, HANGING DROP, temperature 273K |